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Abstract

Background: Male infertility is complex and accounts for approximately 50% of couples facing conception challenges. Factors such as body mass index, age, and sperm parameters play significant roles. Recognizing their impact is vital for diagnosis and treatment. Aim of the study: The study aimed to evaluate DNA integrity in individuals with unexplained infertility and assess its correlation with age, body mass index, and semen parameters. Materials and Methods: In this study, 111 males who met the inclusion criteria were recruited for our study. Selected participants were grouped according to their age, body mass index, and semen parameters, including (sperm count, percentage motility, and morphology) with Aniline Blue Staining and Sperm Chromatin Dispersion Staining used in the IVF center-Baghdad. Results: Our analysis found no significant correlations between Aniline Blue Stain and several parameters, including male age (p = 0.382), body mass index (p = 0.238), sperm concentration (p = 0.117), sperm morphology (p = 0.229), and sperm motility (p = 0.079). Thus, within our study, Aniline Blue Stain showed no statistically significant relationships with these factors. In contrast, Sperm Chromatin Dispersion Staining exhibited significant associations with sperm concentration (p = 0.025) and sperm morphology (p = 0.039) in our study population. However, male age (p = 0.104), body mass index (p = 0.102), and sperm motility (p = 0.173) did not demonstrate significant correlations with Halo Sperm Staining. Conclusion: Evaluating sperm DNA damage through semen analysis is vital in male fertility screening, particularly for unexplained infertility for sperm parameters (sperm concentration and sperm morphology).

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